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Cancer Meeting Presentation 9-14-11

2015-01-13

azim58 - Cancer Meeting Presentation 9-14-11 This presentation was about the PCANTAB and rCompTT vectors for the phage library, protein detection, printing protein lysate onto different slide types, and a mention of the T cell assay gemini project. C:\kurt\storage\CIM Research Folder\kwhittem\Presentations\2011\9-14-11 Cancer Meeting Presentation Notes for Improvement: When cloning scFv into PCANTAB * understand and read about i j ratios * just use 3X more insert not 6X more insert otherwise different inserts can ligate to eachother * use calf intestinal phosphotase instead of shrimp phosphotase (follow protocol or titrate to see how little I need or don't use it at all) * precipitate with tRNA instead of glycogen * put a stuffer into the PCANTAB so that I can separate cut from uncut plasmid o Constructing PCANTAB-pRSET plasmid
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