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Basic plan for random hexamer library construction 1-24-13

2014-08-29

azim58 - Basic plan for random hexamer library construction 1-24-13 I will construct the library using four different methods and then compare the complexity of each method. for primers used see cDNA Library Random Hexamer Plan 12-12-12 Synthesize 1st strand cDNA from poly A purified RNA using reverse transcriptase in In-Fusion SMARTer Directional cDNA Library Construction Kit -> 2nd strand synthesis with PrimeStar Synthesize 1st strand cDNA from poly A purified RNA using reverse transcriptase in In-Fusion SMARTer Directional cDNA Library Construction Kit -> 2nd strand synthesis with dynazyme Synthesize 1st strand cDNA from poly A purified RNA using PrimeScript reverse transcriptase -> 2nd strand synthesis with PrimeStar Synthesize 1st strand cDNA from poly A purified RNA using PrimeScript reverse transcriptase -> 2nd strand synthesis with dynazyme
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