Background Info for Immunosignature to Antigen Work

2014-08-29

azim58 - Background Info for Immunosignature to Antigen Work

My DoD Breast Cancer Predoctoral Traineeship application has some good
background information about my project.

"F:\kurt\storage\CIM Research
Folder\DR\2013\6-16-13\wiki_download\Identification of Breast Cancer
Antigens through Immunosignatures.doc"

"F:\kurt\storage\CIM Research
Folder\DR\2013\6-16-13\wiki_download\References Cited.odt"

Here's a basic outline of the paper

* Career Goals
  o Improve human health

* Training
  o Courses, mentors, Biodesign environment
  o Peter Lee, Kathryn Sykes

* Research Project
  o Introduction
    - description of antigen discovery platform
    - 2 reagents
      @ antibody-carrying sera
      @ random sequence 20 mer peptides

- SMC1fs positive control
    - eventual cancer vaccine

o Background
    - tumor associated antigen repressed mammary tumors in rats [9]
    - whole cell tumor lysate was immunogenic [10]
    - self tumor mixtures in clinical trials [11]
    - more antigens would be good
    - more specific antigens needed
    - tumor associated antigen examples: HER2/neu, MUC1, MAGE
    - Specific Antigens: frameshifts, chromosomal rearrangements,
      aberrant protein modifications
    - example of tumor specific antigen from lymphoma group [13]
    - learn from immune system (from antibodies)
    - random peptide array can distinguish diseases
    - need to discover original antigen
    - SMC1fs positive control
    - SMC1fs found more in cancer than normal cells
    - plan for SMC1fs sera
    - phage display technology
      @ small antibodies
      @ unlimited amount of phage

- compare sera and library
    - conclusion

o Methods
    - Identification of breast cancer specific immunosignature
      @ format of slides
      @ basic procedure
      @ process for determining informative peptides
      @ different random arrays could be used

- Probing biological samples
      @ random peptide array helps purify antibodies
      @ probe biological space
        # Western blot
        # cDNA library

- Phage Library Approach
      @ Process for making phage library

- Discovering human antigens

o Relevance

Cancer vaccine references

* Vaccination with soluble low-molecular weight tumor-associated
  proteins suppresses chemically-induced mammary tumorigenesis in rats
  o I wasn't able to get an electronic copy of this paper online
    normally, but I did get it from the ILLIAD ASU service
    - "F:\kurt\storage\CIM Research
      Folder\DR\2013\6-16-13\wiki_download\Vaccination with soluble
      low-molecular weight tumour-associated proteins suppresses
      Chemically-induced Mammary Tumorigenesis in Rats.pdf"

* Immunotherapeutic potential of whole tumour cells
  o http://www.springerlink.com.ezproxy1.lib.asu.edu/content/a88nu01yyx
    xjvyrv/fulltext.pdf

* Cancer vaccines: between the idea and the reality
  o http://www.nature.com.ezproxy1.lib.asu.edu/nri/journal/v3/n8/pdf/nr
    i1150.pdf

Shen also has some data to show that a cancer vaccine is effective.
"F:\kurt\storage\CIM Research Folder\DR\2013\6-16-13\wiki_download\cancer
vaccine protection .ppt"

Questions
Have I found a paper that shows that scFv bind the same antigen as the
original antibody? I think I've read this before, but now I don't know
where it is.

azim58wiki: