B16F10

2014-08-29

azim58 - B16F10

Some Documents
"C:\kurt\storage\CIM Research Folder\DR\2013\4-13-13\download\B16 model
protocol-1.pdf"

* "C:\kurt\storage\CIM Research Folder\DR\2013\4-13-13\download\Growing
  B16 Melanoma.pdf"

ATCC Subculture Protocol for B16F10
http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Defa
ult.aspx?ATCCNum=CRL-6475&amp;Template=cellBiology

Protocol:

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA
solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells
under an inverted microscope until cell layer is dispersed (usually
within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking
the flask while waiting for the cells to detach. Cells that are difficult
to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently
pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:10 is recommended
Medium Renewal: Every 2 to 3 days

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stock last seen in our lab near freezer space
rack B Box 5 a6 (lot 322557 12/2/05)
rack K Box 7 B1 (passage 12)

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