Analysis of Top Clones 11-19-12

2014-08-29

azim58 - Analysis of Top Clones 11-19-12

I need to do a more thorough analysis of the top clones found here
8-26-12 Plasmids for verification

I need to know where the start codon is, where the plasmid part ends and
the insert begins, what the gene aligns to, and whether or not the gene
is a mutation or a frameshift.

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11-25-12

Earlier when I determined what the sequence aligns to, I aligned to all
mouse sequences. However, I should have just aligned to the mouse refseq
so that I did not obtain multiple alignments to the same things. I will
look at the analysis again. This time I think I will make a powerpoint
slide for each gene. On it I will have the DNA sequence the gene aligns
to and which frame it is in. I will also capture an image of the
alignment. I will also have which protein sequence it aligns to. I will
also capture an image of the alignment.

Here is a template of the info I want to include
"C:\kurt\storage\CIM Research Folder\DR\2012\11-25-12\cDNA Sequence
Analysis\Alignment Info Template 11-25-12.pptx"

Sequence Info found here
"C:\kurt\storage\CIM Research Folder\DR\2012\11-25-12\cDNA Sequence
Analysis\cDNA Sequence Analysis with source info 11-25-12.pptx"

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