Alternative CTL Assays to CRA

2014-08-29

azim58 - Alternative CTL Assays to CRA

Alternative CTL Assays to CRA
Note: Not all of the papers here are about caspase.

Flow cytometry and apoptosis detection notes/paper

Measurement of CTL-Induced Cytotoxicity The Caspase 3 Assay (2003)
Measurement of CTL-Induced Cytotoxicity The Caspase 3 Assay.pdf
"C:\Users\kurtw_000\Documents\kurt\storage\CIM Research Folder\DR\2014\01-24-2014d0911\01-24-2014d0911\Measurement of CTL-Induced Cytotoxicity The Caspase 3 Assay.pdf"

Measurement (annotated) of CTL-Induced Cytotoxicity The Caspase 3
Assay.pdf
"C:\Users\kurtw_000\Documents\kurt\storage\CIM Research Folder\DR\2014\01-24-2014d0911\01-24-2014d0911\Measurement (annotated) of CTL-Induced Cytotoxicity The Caspase 3 Assay.pdf"

Multiparametric Flow Cytometry 2007

A sensitive flow cytometry-based cytotoxic T-lymphocyte assay through
detection of cleaved caspase 3 in target cells (2005)
http://www.sciencedirect.com/science/article/pii/S0022175905001754

Assessment of Lymphocyte-Mediated Cytotoxicity Using Flow Cytometry (2004)
http://www.springerlink.com/content/j8v4l4gp83241442/#section=98733&amp;pag
e=1
^^Vanessa tried reading this paper but it only lets me read the first 3
pages and won't let me download the PDF. Where you able to get more than
that from this??

Visualization and quantification of cytotoxicity mediated by antibodies
using imaging flow cytometry (2011)
http://www.sciencedirect.com.ezproxy1.lib.asu.edu/science/article/pii/S0022
175911000585
Note: This paper seems to focus more on antibody dependent cellular
cytotoxicity.

Development and application of a multiplexable flow cytometry-based assay
to quantify cell-mediated cytolysis (2010)
http://onlinelibrary.wiley.com.ezproxy1.lib.asu.edu/doi/10.1002/cyto.a.2088
7/abstract;jsessionid=BC08B53F2E7D099049D34E46A0267D3A.d01t02
Note: This paper seems to focus on the quantitative aspects of
cell-mediated cytolysis

Application of a Flow Cytometric Cytotoxicity Assay for Monitoring Cancer
Vaccine Trials (2009)
http://ovidsp.tx.ovid.com.ezproxy1.lib.asu.edu/sp-3.4.2a/ovidweb.cgi?QS2=43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Note: this paper looks quite interesting and very applicable to our
situation
^^I couldn't open this either :(

New Flow cytometric assays for monitoring cell-mediated cytotoxicity
review paper
I may want to
look up justification for sensitivity jam of method

This paper was recommended by Blattman
Counting Antigen Specific CD8 T Cells A Reevaluation of Bystander
Activation during viral infection

azim58wiki: