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Alignment with whole plasmid 070512
2014-08-29
azim58 - Alignment with whole plasmid 070512 reverse_primer ------------------------------------------------------------ pcomb AGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTG 60 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb GCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTA 120 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb GCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGG 180 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb AATTGTGAGCGGATAACAATTGAATTCAGGAGGAATTTAAAATGAAAAAGACAGCTATCG 240 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb CGATTGCAGTGGCACTGGCTGGTTTCGCTACCGTGGCCCAGGCGGCCGAGCTCGCCATGG 300 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb CTGGTTGGGCAGCGAGTAATAACAATCCAGCGGCTGCCGTAGGCAATAGGTATTTCATTA 360 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TGACTGTCTCCTTGGCGACTAGCTAGTTTAGAATTCGTAATCATGGTCATAGCTGTTTCC 420 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTG 480 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCC 540 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb CGCTTTCCAGTCGGGAAACCTGTCGTGTTACTAATGATGGTGATGGTGATGGCTAGTTTT 600 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb GTCACAAGATTTGGGCTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCAC 660 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb GTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCTGAG 720 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb GGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC 780 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb GCCTGAGTTCCACGACACCGTCGCCGGTTCGGGGAAGTAGTCCTTGACCAGGCAGCCCAG 840 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb GGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGGGGAAGACCGATGGGCC 900 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb CTTGGTGGAGGCTGCGGAGACGGTGACCGTGGTACCAGCAGAAACCTGGCCAGGCTCCCA 960 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb GGCTCCTCATCTATGGTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCA 1020 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb GTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAG 1080 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TGTACTACTGTCAGCAGTATGGTGGCTCACCGTGGTTCGGCCAAGGGACCAAGGTGGAAC 1140 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA 1200 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb AATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAG 1260 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGC 1320 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb AGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT 1380 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb ACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTTGCCCGTCA 1440 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb CAAAGAGCTTCAACAGGGGAGAGTGTTAGTTCTAGATAATTAATTAGGAGGAATTTAAAA 1500 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TGAAATACCTATTGCCTACGGCAGCCGCTGGATTGTTATTACTCGCTGCCCAACCAGCCA 1560 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TGGCCGAGGTGCAGCTGCTCGAGATGAGCGATAAAATTATTCACCTGACTGACGACAGTT 1620 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TTGACACGGATGTACTCAAAGCGGACGGGGCGATCCTCGTCGATTTCTGGGCAGAGTGGT 1680 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb GCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCA 1740 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb AACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCA 1800 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGG 1860 reverse ------------------------------------------------------------ forward ---------------------------------------------------NNNNNNNNG 9 reverse_primer ------------------------------------------------------------ pcomb GTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTACCCGTACG 1920 reverse ------------------------------------------------------------ forward NNGNNNTGNCTAN-GGNCAGTTGAA-GAGTTCCTCGACGCTAACCTGGCGTACCCGTACG 67 reverse_primer ------------------------------------------------------------ pcomb ACGTTCCGGACTACGGTTCTACTAG-TGGCCAG------GCCGGCCAGCACCATCACCAT 1973 reverse ---------------------------------------NNTTNNTAGCACCNTCACCAT 21 forward ACGTTCCGGACTACGGTTCTACTAGGCGGCCGCTTTTTTTTTTTTTAGCACCATCACCAT 127 reverse_primer ------------------------------------------------------------ pcomb CACCATGGC-GCATACCCGTACGACGTTCCGGACTACGCTTCTTAGGAGGGTGGTGGCTC 2032 reverse CANCATGGNCGCATACCCGTACGACGTTCCGGACTACGCTTCTTAGGAGGGTGGTGGCTC 81 forward CACCATGGC-GCATACCCGTACGACGTTCCGGACTACGCTTCTTAGGAGGGTGGTGGCTC 186 reverse_primer ------------------------------------------------------------ pcomb TGAGGGTGGCGGTTCTGAGGGTGGCGGCTCTGAGGGAGGCGGTTCCGGTGGTGGCTCTGG 2092 reverse TGAGGGTGGCGGTTCTGAGGGTGGCGGCTCTGAGGGAGGCGGTTCCGGTGGTGGCTCTGG 141 forward TGAGGGTGGCGGTTCTGAGGGTGGCGGCTCTGAGGGAGGCGGTTCCGGTGGTGGCTCTGG 246 reverse_primer ------------------------------------------------------------ pcomb TTCCGGTGATTTTGATTATGAAAAGATGGCAAACGCTAATAAGGGGGCTATGACCGAAAA 2152 reverse TTCCGGTGATTTTGATTATGAAAAGATGGCAAACGCTAATAAGGGGGCTATGACCGAAAA 201 forward TTCCGGTGATTTTGATTATGAAAAGATGGCAAACGCTAATAAGGGGGCTATGACCGAAAA 306 reverse_primer ------------------------------------------------------------ pcomb TGCCGATGAAAACGCGCTACAGTCTGACGCTAAAGGCAAACTTGATTCTGTCGCTACTGA 2212 reverse TGCCGATGAAAACGTGCTACAGTCTGACGCTAAAGGCAAACTTGATTCTGTCGCTACTGA 261 forward TGCCGATGAAAACGTGCTACAGTCTGACGCTAAAGGCAAACTTGATTCTGTCGCTACTGA 366 reverse_primer ------------------------------------------------------------ pcomb TTACGGTGCTGCTATCGATGGTTTCATTGGTGACGTTTCCGGCCTTGCTAATGGTAATGG 2272 reverse TTACGGTGCTGCTATCGATGGTTTCATTGGTGACGTTTCCGGCCTTGCTAATGGTAATGG 321 forward TTACGGTGCTGCTATCGATGGTTTCATTGGTGACGTTTCCGGCCTTGCTAATGGTAATGG 426 reverse_primer ------------------------------------------------------------ pcomb TGCTACTGGTGATTTTGCTGGCTCTAATTCCCAAATGGCTCAAGTCGGTGACGGTGATAA 2332 reverse TGCTACTGGTGATTTTGCTGGCTCTAATTCCCAAATGGCTCAAGTCGGTGACGGTGATAA 381 forward TGCTACTGGTGATTTTGCTGGCTCTAATTCCCAAATGGCTCAAGTCGGTGACGGTGATAA 486 reverse_primer ------------------------------------------------------------ pcomb TTCACCTTTAATGAATAATTTCCGTCAATATTTACCTTCCCTCCCTCAATCGGTTGAATG 2392 reverse TTCACCTTTAATGAATAATTTCCGTCAATATTTACCTTCCCTCCCTCAATCGGTTGAATG 441 forward TTCACCTTTAATGAATAATTTCCGTCAATATTTACCTTCCCTCCCTCAATCGGTTGAATG 546 reverse_primer ------------------------------------------------------------ pcomb TCGCCCTTTTGTCTTTAGCGCTGGTAAACCATATGAATTTTCTATTGATTGTGACAAAAT 2452 reverse TCGCCCTTTTGTCTTTGGCGCTGGTAAACCATATGAATTTTCTATTGATTGTGACAAAAT 501 forward TCGCCCTTTTGTCTTTGGCGCTGGTAAACCATATGAATTTTCTATTGATTGTGACAAAAT 606 reverse_primer ------------------------------------------------------------ pcomb AAACTTATTCCGTGGTGTCTTTGCGTTTCTTTTATATGTTGCCACCTTTATGTATGTATT 2512 reverse AAACTTATTCCGTGGTGTCTTTGCGTTTCTTTTATATGTTGCCACCTTTATGTATGTATT 561 forward AAACTTATTCCGTGGTGTCTTTGCGTTTCTTTTATATGTTGCCACCTTTATGTATGTATT 666 reverse_primer --------TTGCTAACATACTGCGTAATAAGGAG-------------------------- 26 pcomb TTCTACGTTTGCTAACATACTGCGTAATAAGGAGTCTTAAGCTAGCTAATTAATTTAAGC 2572 reverse TTCTACGTTTGCTAACATACTGCGTAATAAGGAGGCC-------GCAGATCTGCTCT--C 612 forward TTCTACGTTTGCTAACATACTGCGTAATAAGGAGGCC-------GCAGATCTGCTCT--C 717 ************************** reverse_primer -GCCGCAGATCTGGGAAATTG--------------------------------------- 46 pcomb GGCCGCAGATCTGGGAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTT 2632 reverse TGAGGAGGATCTGGGAAATNGTAAGCGTTAATATTTTNTTAAAATNCGCNNNNAANNNNN 672 forward TGANGAGGATCTGGGAAATTGTAAGCGTTAATATTTTGNTAAAATTCGCGTTAAATTTTT 777 * * ************ * reverse_primer ------------------------------------------------------------ pcomb GTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAA 2692 reverse NNNNNN------------------------------------------------------ 678 forward GTTAANTCAGCTCATTTTTTAACCAATNNNCCGAAATCGNNAAA-TCCCTTATAAATCAA 836 reverse_primer ------------------------------------------------------------ pcomb AAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAA 2752 reverse ------------------------------------------------------------ forward A-GAATANACCGANATANGGTTNAGTGTTGTNCN-GTTTGNNACNN-ANNCCACTATNNN 893 reverse_primer ------------------------------------------------------------ pcomb AGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTAC 2812 reverse ------------------------------------------------------------ forward N-AANGTGGANTCCAANNGNNNNNNNNNNANN-CNGTCTATCNNGNNNANGGCNNNNAN- 950 reverse_primer ------------------------------------------------------------ pcomb GTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGA 2872 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb ACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAA 2932 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb AGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGC 2992 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCAGGTGGCA 3052 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb CTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATA 3112 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGA 3172 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb GTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTC 3232 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb CTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTG 3292 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb CACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCC 3352 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb CCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTAT 3412 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb CCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACT 3472 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAAT 3532 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGA 3592 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCC 3652 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGA 3712 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAG 3772 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb CTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGC 3832 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb GCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGT 3892 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb CTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCT 3952 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb ACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTG 4012 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb CCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTG 4072 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb ATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCA 4132 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGA 4192 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAA 4252 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb AACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGA 4312 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb AGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGT 4372 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGT 4432 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGAT 4492 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb AGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCT 4552 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCA 4612 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb CGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAG 4672 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb AGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTC 4732 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb GCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGA 4792 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb AAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACA 4852 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb TGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAG 4912 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer ------------------------------------------------------------ pcomb CTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGG 4972 reverse ------------------------------------------------------------ forward ------------------------------------------------------------ reverse_primer - pcomb A 4973 reverse - forward -
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