8-26-12 Plasmids for verification

2014-08-29

azim58 - 8-26-12 Plasmids for verification

082612 Plasmids for verification

Here is the key for the plasmids I would like to select to produce
protein from

Well from 16E3 Library Colony number (1-10) Plasmid Number ID Given to
Plasmid
"21E11" 4 4 082612_4
22H10 1 11 082612_11
21G4 1 21 082612_21
11G12 1,3 31,33 082612_31,082612_33
7F12 1,2 41,42 082612_41,082612_42
"7E12" 1 51 082612_51
10A5 1,2 61,62 082612_61,082612_62
13A5 1 71 082612_71
29A5 1 81 082612_81

I will need to transform these plasmids into e coli. Pick a colony for
overnight culture, and then produce the protein.

These plasmids are located here: Selected colony Plasmids from 16E3
library 8-13-12 KW at box in -20 C nearest to benches

see also Sent selected colony plasmids from 16E3 library for sequencing
8-13-12

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11-19-12
Note a better table than the one found on this page (one with stop codon
information and blast information) can be found here.

"C:\kurt\storage\CIM Research Folder\DR\2012\11-19-12\sequence
analysis\8-26-12 plasmid details.xlsx"
Sequencher file
"C:\kurt\storage\CIM Research Folder\DR\2012\9-10-12\tumor lib
sequences\Analysis of sequences for stop codons 2 9-10-12.SPF"

Here are a few of the sequences represented on the "8-26-112 plasmid
details.xlsx" table: Rps8, Eif1a, Rnf130, Cbx3, Hiatl1, Sec61b, Wfdc17

Ordered primers for validation 11-19-12

actually,
see powerpoint here
"C:\kurt\storage\CIM Research Folder\DR\2012\11-25-12\cDNA Sequence
Analysis\cDNA Sequence Analysis with source info 2 11-25-12.pptx"
also
"C:\kurt\storage\CIM Research Folder\DR\2012\11-25-12\cDNA Sequence
Analysis\cDNA sequence analysis clean 12-11-12.pptx"
(other files such as annotated ape files found here)
C:\kurt\storage\CIM Research Folder\DR\2012\11-25-12\cDNA Sequence
Analysis

Information about some of the proteins in the final screen
"C:\kurt\storage\CIM Research Folder\DR\2013\3-15-13\Information about
some of the proteins in the final screen.docx"

versions of protein sequences from associated transcript sequences

===========================================================================
9-10-12
I did some earlier analysis on 9-10-12, but I was not able to reproduce
all of the BLAST results with this analysis. For example I could not
reproduce the DEAD box match
Analysis of sequences for stop codons 9-10-12
"C:\kurt\storage\CIM Research Folder\DR\2012\9-10-12\tumor lib
sequences\Analysis of sequences for stop codons 9-10-12.docx"
"C:\kurt\storage\CIM Research Folder\DR\2012\9-10-12\tumor lib
sequences\Analysis of sequences for stop codons 9-10-12.xlsx"

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