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7-AAD or PI protocol

2014-08-29

azim58 - 7-AAD or PI protocol 7-AAD or PI protocol Before starting: Dilute 10X binding buffer to 1X using distilled water (1mL 10X binding buffer + 9mL dH20) 1. Wash cells once in PBS 2. Wash cells once in 1X binding buffer (prepared above) 3. Resuspend cells in 1X binding buffer at 1-5x10^6/mL 4. Add 5uL of PI staining solution OR 7-AAD staining solution. 5. Analyze by flow cytometry within 4 hours, storing at 2-8C in the dark Note that to run through our flow cytometer, should add 1X PBS right before analysis so that the final volume is 1mL (as preferred by the cytometer) Documents
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