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3-25-13 sequence investigation
2014-08-29
azim58 - 3-25-13 sequence investigation Mara sent our pcomb plasmids for sequencing with SP6, SP7, and SP8. None of the sequences came back well. <sequencing results> e-mail https://mail.google.com/mail/u/0/?ui=2&shva=1#label/Career/13d4b4670179 cc87 https://mail.google.com/mail/u/0/?ui=2&shva=1#label/Career/13d9a0e7f270 1231 </sequencing results> sequencher file of sequencing results "C:\kurt\storage\CIM Research Folder\DR\2013\3-25-13\pcomb\pcomb sequencing results.SPF" raw pcomb sequencing files "C:\kurt\storage\CIM Research Folder\DR\2013\3-25-13\pcomb\raw pcomb sequencing files" Should the primers have aligned with the expected pcomb3xssv3 sequence? Yes they should have. Here is a sequencher file showing 100% alignment. "C:\kurt\storage\CIM Research Folder\DR\2013\3-25-13\pcomb\alignment of 6 7 and 8 with expected pcomb3xssv3.SPF"
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