azim58 - smc_fus primer


smc_fus

CACTGCACCCAGAGCCATTG
Tm of 55.8
20 bp

smc_fus+pSMART2IF forward screening primer produce an approximately 307
bp fragment if the smc1afs sequence is oriented in the forward orientation
307 bp sequence
TCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCAAGC
AGTGGTATCAAGCGAGGCTTACTGTACACCCGGCTTGCACTGCGCTATTTCCCGCGCGGGCTAACCTCGTTCCCA
GGAGTAAGGCGCGGCCTTTCCTACGCCAGCGCCGCTGTCATGGGTTTCCTGAAACTGATTGAGATCGAGAACTTT
AAATCGTACAAGGGTCGACAGATTATCGGACCATTTCAGAGGTTTACCGCCATCATTGGACCCAATGGCTCTGGG
TGCAGTG

smc_fus+pSMART2IF reverse sequencing primer produce an approximate 363 bp
fragment when the smc1afs is in the vector in the reverse orientation
363 bp fragment
CACTGCACCCAGAGCCATTGGGTCCAATGATGGCGGTAAACCTCTGAAATGGTCCGATAATCTGTCGACCCTTGT
ACGATTTAAAGTTCTCGATCTCAATCAGTTTCAGGAAACCCATGACAGCGGCGCTGGCGTAGGAAAGGCCGCGCC
TTACTCCTGGGAACGAGGTTAGCCCGCGCGGGAAATAGCGCAGTGCAAGCCGGGTGTACAGTAAGCCTCGCTTGA
TACCACTGCTTCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCG
TTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGG

products from some primer pairs
-smc_fus plus SMC1-mou-FWD




pSMART2IF vector with SMC1afs sequence in forward orientation


pSMART2IF vector with SMC1afs sequence in reverse orientation

"F:\kurt\storage\CIM Research Folder\DR\2013\6-16-13\smc1fs
screen\psmart2IF with smc1afs rc.SPF"
or
"F:\kurt\storage\CIM Research Folder\DR\2013\6-16-13\smc1fs
screen\psmart2IF with smc1afs rc.SPF"