azim58 - sequencing results 7-22-13


I was hoping to find out whether the smc_fus sequence in sample "87" was
in-frame. However, only two of the sequencing results were any good. They
assembled together, but this sequence had nothing to do with smc or the
primers that I used. I think that the pjet cloning and transformation did
not go well since there were so few colonies.

sequencing results found here
"F:\kurt\storage\CIM Research Folder\DR\2013\7-22-13\sequencing data
7-22-13"


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They reloaded the forward sequence, but there were only 156 good bp. The
smc_fus primer kind of aligned with some of these base pairs, but it is
hard to tell whether this is a true alignment. When I blasted the
sequence, these base pairs completely align with 68% of the Hnrnpab
transcript.

F:\kurt\storage\CIM Research Folder\DR\2013\7-28-13\potential smc_fus
fragment analysis


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I sent the cloned pJET miniprepped fragment for sequencing, and this
sequence aligned with the Hnrnpab transcript once again.
"F:\kurt\storage\CIM Research Folder\DR\2013\7-29-13\potential smc_fus
sequences 7-29-13"

I may try to screen my pools using nested PCR in an attempt to get more
successful amplification.