pure text notes on construction of human tumor cDNA section 8-30-13

2014-08-29

++ pure text notes on construction of human tumor c DNA section 8-30-13

info for "Human breast tumor RNA characteristics" table
"F:\kurt\storage\CIM Research Folder\kwhittem\Presentations\2012\General Meeting Presentation 10-24-12\version with links to info\General Meeting Presentation 10-24-12.pptx"

info for "Tumor cDNA synthesized from random hexamer primers" figure
"S:\Research\Cancer_Eradication\Discovering tumor specific antigens\Tumor cDNA Library\9.19.12\ds htcDNA optimization 9-19-12.svg"
"F:\kurt\storage\CIM Research Folder\kwhittem\Presentations\2012\General Meeting Presentation 10-24-12\version with links to info\General Meeting Presentation 10-24-12.pptx"

RNA integrity check before random pentadecamer library construction
-"F:\kurt\storage\CIM Research Folder\DR\2013\2-22-13\RNA\rna integrity test 2-21-13.png"
-"F:\kurt\storage\CIM Research Folder\kwhittem\Presentations\2013\Spring 2013 Committee Meeting\Spring 2013 Committee Meeting Presentation 5-20-13.pptx"

Second strand synthesis with random pentadecamer primers
-F:\kurt\storage\CIM Research Folder\DR\2013\3-27-13\random cDNA library
-"F:\kurt\storage\CIM Research Folder\kwhittem\Presentations\2013\Spring 2013 Committee Meeting\Spring 2013 Committee Meeting Presentation 5-20-13.pptx"

Gel fragments pre and post electroelution
"S:\Research\Cancer_Eradication\Discovering tumor specific antigens\Tumor cDNA Library\3-27-13 cdna library\samples 1 2 and 7 pre-electroelution.jpg“
"S:\Research\Cancer_Eradication\Discovering tumor specific antigens\Tumor cDNA Library\3-28-13\3-28-13 post electroelution.jpg"
-"F:\kurt\storage\CIM Research Folder\kwhittem\Presentations\2013\Spring 2013 Committee Meeting\Spring 2013 Committee Meeting Presentation 5-20-13.pptx"

cDNA library after electroelution and precipitation
"F:\kurt\storage\CIM Research Folder\kwhittem\Presentations\2013\Spring 2013 Committee Meeting\Spring 2013 Committee Meeting Presentation 5-20-13.pptx"

cDNA library after electroelution, ethanol precipitation, in-fusion, and ethanol precipitation
-F:\kurt\storage\CIM Research Folder\DR\2013\4-19-13\electroeluted library\gel of electroeluted library 4-19-13.png
-"F:\kurt\storage\CIM Research Folder\kwhittem\Presentations\2013\Spring 2013 Committee Meeting\Spring 2013 Committee Meeting Presentation 5-20-13.pptx"

Electroporation condition test
-F:\kurt\storage\CIM Research Folder\DR\2013\5-10-13\Transformation Results 5-10-13.xlsx
-"F:\kurt\storage\CIM Research Folder\kwhittem\Presentations\2013\Spring 2013 Committee Meeting\Spring 2013 Committee Meeting Presentation 5-20-13.pptx"

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